The service has some pre-design in silico to guarantee the optimal enzyme selection on the preparation of the library.
● Sequencing on NovaSeq with PE150.
● Library preparation with double barcoding, enabling pooling of over 1000 samples.
● Independent of reference genome:
With reference genome: SNP and InDel discovery
Without reference genome: sample clustering and SNP discovery
● In the in-silico pre-design stage multiple restriction enzyme combinations are screened to find the ones that generate a uniform distribution of SLAF tags along the genome.
● During the pre-experiment, three enzyme combinations are tested in 3 samples to generate 9 SLAF libraries, and this information is used to choose the optimal restriction enzyme combination for the project.
Container: 2 ml centrifuge tube
(For most of the samples, we recommend not to preserve in ethanol)
Sample labeling: Samples need to be clearly labeled and identical to submitted sample information form.
Shipment: Dry-ice: Samples need to be packed in bags first and buried in dry-ice.
