De novo
This technique is particularly useful with challenging genomes such as those with high degree of heterozygosity, repetitive regions, polypoloid genomes, abnormal CG contents etc.
Our one-stop solution provides integrated sequencing services and bioinformatic analysis that deliver a high-quality de novo assembled genome. An initial genome survey with Illumina provides estimations of genome size and complexity, and this information is used to guide the next step of long-read sequencing with PacBio HiFi, followed by de novo assembly of contigs. The subsequent use of HiC assembly enables anchoring of the contigs to the genome, obtaining a chromosome-level assembly. Finally, the genome is annotated by gene prediction and by sequencing expressed genes, resorting to transcriptomes with short and long reads.
-- Integration of multiple sequencing and bioinformatic services in a one-stop solutionService suitable for the constructing novel
-- genomes or improvement of existing reference genomes for species of interest.
Resequencing
-- Library preparation can be standard or PCR-free
-- Available in 4 sequencing platforms: Illumina NovaSeq, MGI T7, Nanopore Promethion P48 or PacBio Revio.
-- Bioinformatic analysis focused on variant calling: SNP, InDel, SV and CNV
● Extensive Expertise and Publication Record: For de novo services, we have accumulated massive experience in high-quality genome assembly of diverse species, including diploid genomes and highly complex genomes of polyploid and allopolyploid species. Since 2018, we have contributed to over 300 high-impact publications, and 20+ of them are published in Nature Genetics. On genome re-sequecning, we accumulated over 1000 species, which resulted in over 1000 published cases with a cumulative impact factor of over 5000.
● One-stop Solution: On de novo sequencing, our integrated approach combines multiple sequencing technologies and bioinformatic analyses into a cohesive workflow, delivering a high-quality assembled genome.
● Tailored to Your Needs: Our service workflow is customizable, allowing adaptation for genomes with diverse features and specific research needs.
● Highly Skilled Bioinformatics and Laboratorial Team: Whether it is for de novo sequencing or re-sequencing, our team has a skilled set of tools and knowledge to guarantee the project success. This can be corroborated with the series of patents and software copyrights they’ve developed.
● Post-Sales Support: Our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
● Comprehensive Bioinformatics Analysis: Including variation calling and function annotation.
● Comprehensive Annotation for Sequencing: We use multiple databases to functionally annotate the genes with identified variations and perform the corresponding enrichment analysis, providing insights on your research projects.