Service Features
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Genome-wide variant detection with comprehensive coverage of coding and non-coding regions.
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Sequencing with multiple advanced platforms—Illumina, MGI, and long-read technologies (PacBio and Nanopore)—offering flexible options for diverse needs.
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Detects and annotates variant sites across individuals, delivering insights into genetic differences.
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Specialized bioinformatics pipeline for tumor and genetic disease research.
Service Advantages
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Extensive Project Experience: Extensive experience supporting projects across diverse research fields.
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Rigorous Quality Control: Stringent quality control throughout sample processing, library preparation, sequencing, and data analysis ensures reliable, high-quality results.
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Experienced Bioinformatics Team and Standardized Workflow: Expert analysts and optimized, standardized workflows enable accurate and efficient data interpretation.
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Customized Bioinformatics Analysis: Flexible, tailored analytical solutions address specific research objectives and deliver in-depth insights aligned with project-specific needs.
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Three-Month Post-Delivery Support: Three months of support following result delivery, including assistance with result interpretation, follow-up consultations, and troubleshooting.
Sample Specifications
| Type |
Concentration and Purity |
Agarose Gel |
Conc.
(ng/μl) |
Amount |
OD
260/280 |
OD
260/230 |
Nanodrop/Quibit |
WGS-FF*
NGS |
≥ 1.0 |
≥ 30 ng |
- |
- |
- |
No or limited Contamination.
No or with degradation (the main band is clear).
FFPE DNA: If the main band is unclear, a distinct smear above 2kb is required.
|
WGS-FFPE*
NGS |
≥ 5.0 |
≥ 50 ng |
- |
- |
- |
WGS NGS
PCR-FREE |
≥ 40 |
≥ 500 ng |
- |
- |
- |
WGS
ONT |
≥ 40 |
≥ 2.0 μg |
1.7-2.2 |
1.5-3.0 |
0.8-2.5 |
WGS
PB |
≥ 50 |
≥ 8.0 μg |
1.7-2.2 |
0.8-2.5 |
0.8-2.5 |
Notes:
FF: Fresh-frozen samples.
FFPE: Formalin-fixed, paraffin-embedded samples.
Sample Requirements
| Tissue or Extracted DNA |
Illumina/MGI |
Nanopore |
PacBio |
| Muscle |
0.5–1 g |
≥ 3.5 g |
≥ 3.5 g |
| Blood |
1.5 mL |
≥ 0.5 mL |
≥ 5 mL |
| Cultured Cells |
≥ 1 × 106 |
≥ 1 × 107 |
≥ 1 × 108 |
| FFPE Sample |
|
|
|
| Extracted DNA |
Regular Library Preparation
Concentration: ≥ 1 ng/μL
Amount: ≥ 30 ng
Limited or no degradation
or contamination
|
Concentration: ≥ 40 ng/μL
Amount: ≥ 4 μg/flow cell/sample
OD 260/280: 1.7–2.2
OD 260/230: ≥ 1.5
Limited or no degradation
or contamination
|
Concentration: ≥ 50 ng/μL
Amount: ≥ 10 μg/flow cell/sample
OD 260/280: 1.7–2.2
OD 260/230: 1.8–2.5
Limited or no degradation
or contamination
|
PCR-free Library Preparation
Concentration: ≥ 40 ng/μL
Amount: ≥ 500 ng
Limited or no degradation
or contamination
|
DNA Extracted from FFPE Samples
Concentration: ≥ 5 ng/μL
Fragments longer than 2 kb
|
- |
- |
Service Work Flow
Sample delivery
DNA extraction
Library construction
Sequencing
Data analysis
Data delivery