● Platform: MGI-DNBSEQ-T7
● Sequencing modes: PE150
● Transfer of Illumina libraries to MGI: enabling sequencing of high data volumes at low cost.
● Quality control of libraries before sequencing.
● Sequencing data QC and delivery: delivery of QC report and raw data in fastq format after demultiplexing and filtering Q30 reads.
● Versatility of Sequencing services: the customer may choose to sequence by lane or amount of data.
● High data output: 1500 Gb/lane
● Delivery of sequencing QC report: with quality metrics, data accuracy and overall performance of the sequencing project.
● Mature sequencing process: with short turn-around time.
● Rigorous Quality Control: we implement strict QC requirements to guarantee the delivery of consistently high-quality results.
|
Data Amount (X) |
Concentration (qPCR/nM) |
Volume |
|
|
Partial Lane
|
X ≤ 10 Gb |
≥ 1nM |
≥ 25 μl |
|
10 Gb < X ≤ 50 Gb |
≥ 2 nM |
≥ 25 μl |
|
|
50 Gb < X ≤ 100 Gb |
≥ 3 nM |
≥ 25 μl |
|
|
X > 100 Gb |
≥ 4 nM |
||
|
Single Lane |
Per Lane |
≥ 1.5 nM / Library pool |
≥ 25 μl / Library pool |
In addition to concentration and total amount, a suitable peak pattern is also required.
Note: Lane sequencing of low diversity libraries requires PhiX spike-in to ensure robust base calling.
We recommend submitting pre-pooled libraries as samples. If you require BMKGENE to perform library pooling, please refer to the library requirements for partial lane sequencing.
Main peak should be within 300-450 bp.
Libraries should have a single main peak, no adapter contamination and no primer dimers.