Illumina pre-made libraries

Features

● Platforms: Illumina NovaSeq 6000 and NovaSeq  X Plus

● Sequencing modes: PE150 and PE250

● Quality control of libraries before sequencing

● Sequencing data QC and delivery: delivery of QC report and raw data in fastq format after demultiplexing and filtering Q30 reads

 

 

Service Advantages

Sample Platforms

Platform	Flow Cell	Sequencing mode	Unit	Estimated output
NovaSeq X	10B (8 lanes)	PE150	Single Lane Partial Lane	375Gb / Lane
	25B ( 8 lanes)	PE150	Single Lane Partial Lane	1000 Gb/Lane
NovaSeq 6000	SP Flow cell (2 lanes)	PE250	Flow Cell Single Lane Partial Lane	325-400 M reads / Lane
	S4 Flow cell (4 lanes)	PE150	Flow Cell Single Lane Partial Lane	~800 Gb / Lane

Sample Requirements

	Data Amount (Gb)	Conc. (qPCR) PE150	Conc. (qPCR) PE250	Volume
Partial Lane	G ≤ 10	≥ 1 nM	≥ 2nM	≥ 25 μl
	10 < G ≤ 50	≥ 2 nM	≥ 3nM	≥ 25 μl
	50 < G ≤ 100	≥ 3 nM	≥ 4nM	≥ 25 μl
	G > 100	≥ 4 nM	≥ 4nM	≥ 25 μl
Single Lane	Per Lane	≥ 1.5 nM/Library pool	≥ 2 nM/Library pool	≥ 25 µL/Library pool

In addition to concentration and total amount, a suitable peak pattern is also required.

Note: Lane sequencing of low diversity libraries requires PhiX spike-in to ensure robust base calling.

           We recommend submitting pre-pooled libraries as samples for lane sequencing. If you require BMKGENE to perform library pooling, please refer to the library requirements for partial lane sequencing.

Library Size (Peak map)

For PE150 sequencing, the insert size should be 300–400 bp (excluding adapters); for PE250 sequencing, the insert size should be 150–500 bp (excluding adapters).
Libraries should have a single main peak, no adapter contamination and no primer dimers.

Please reach out to us if your samples don't meet the starting material requirements.

Contact us

Service Workflow

Library quality control

Sequencing

Data quality control

Project delivery