OEM China Dnbseq - PacBio-Full-length Transcriptome (Non-Reference) – Biomarker

Raw data procession

CCS extraction Full-length non-chimeric(FLNC) reads identification Consensus transcripts identification

Bioinformatics

Data quality control Protein-protein interaction Expression Alternative splicing Go enrichment

Transcipt identification

Unigenes construction Isoform identification Alternative splicing Differential alternative splicng APA analysis Differential APA Fusion genes Differential fusion gene Homologuos genes Gene family SSR Gene structure optimization SNP analysis lncRNA prediction lncRNA target prediction CDS prediction Transcription factor

Expression quantification

Differential expression analysis (on genes and on transcripts)

Functional annotation

Functional enrichment Protein-protein interaction network