Small RNA Sequencing-Illumina

Features

● Library preparation includes a size selection step

● Bioinformatic analysis centered around miRNA prediction and their targets

Service Advantages

Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

● Plants:

   Root, Stem or Petal: 450 mg

   Leaf or Seed: 300 mg

   Fruit: 1.2 g

● Animal:

   Heart or Intestine: 450 mg

   Viscera or Brain: 240 mg

   Muscle: 600 mg

   Bones, Hair or Skin: 1.5g

● Arthropods:

   Insects: 9g

   Crustacea: 450 mg

● Whole blood: 2 tubes

● Cells: 10⁶ cells

● Serum and Plasma: 6 mL

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3.

Shipment:

1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.

2. RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

Experiment design

Sample delivery

RNA extraction

Library construction

Sequencing

Data analysis

After-sale services