Latest successful cases on Nanopore RNA-Sequencing with Biomarker Technologies
Nanopore-based Full-length Transcriptome Sequencing
Nanopore sequencing distinguishes itself from other sequencing platforms, in that the nucleotides are read directly without DNA synthesis and generates long reads at tens of kilobases. This empowers direct read-out of full-length transcripts and tackling the challenges in transcriptomic studies.
√ Low sequence-specific bias
√ Full-length readout of cDNA to for gene structure studies
√ Less data required to cover same number of transcripts
√ Identification of multiple isoforms per gene
√ Expression quantification on isoform-level
Typical Bioinformatics Pipeline
Latest Successful Cases with Biomarker Technologies
1. Comparative analyses of full-length transcriptomes reveal Gnetum luofuense stem developmental dynamics
Journal: Frontiers in Genetics
Published: Mar. 2021
Keywords: MinION | Alternative splicing | APA | lncRNA | Differentially expressed transcripts
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2. Unidirectional movement of small RNAs from shoots to roots in interspecific heterografts
Journal: Nature Plants
Published: Jan. 2021
Keywords: Nanopore | Illumina | full-length mobile mRNA detection
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3. Full-length transcriptome analysis of asparagus roots reveals the molecular mechanism of salt tolerance induced by arbuscular mycorrhizal fungi
Journal: Environmental and Experimental Botany
Published: Jan. 2021
Keywords: MinION | Differentially expressed transcripts | Transcription factors | Alternative splicing
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4. Physiological Characteristic Changes and Full-Length Transcriptome of Rose (Rosa chinensis) Roots and Leaves in Response to Drought Stress
Journal: Plant and Cell Physiology
Published: Oct. 2020
Keywords: PromethION | Differentially expressed transcripts | Functional prediction | Spliced isoforms | TF and lncRNA
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5. Analysis and comprehensive comparison of PacBio and nanopore-based RNA sequencing of the Arabidopsis transcriptome
Journal: Plant Method
Published: Apr. 2020
Keywords: GridION | PromethION | PacBio Sequel | Illumina NovaSeq | Genome alignment | Transcript identification | Alternative splicing | SSR | LncRNA | Isoform quantification
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News and Highlights aims at sharing the latest successful cases with Biomarker Technologies, capturing novel scientific achievements as well as prominent techniques applied during the study.