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Manufacturer of 16s Rrna Sequencing Service - circRNA sequencing – Illumina – Biomarker

Service Advantages

Sample Requirements and Delivery

Sample Requirements:

Nucleotides:   

Purity Integrity Contamination Amount
OD260/280≥1.7-2.5;OD260/230≥0.5-2.5; For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation Limited or no protein or DNA contamination shown on gel. Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended.

Blood samples:
PA×geneBloodRNATube;  
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)

Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3... ...

Shipment:
Dry-ice: Samples need to be packed in bags and buried in dry-ice.
RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

Experiment design

Sample delivery

RNA extraction

Library construction

Sequencing

Data analysis

After-sale services


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