üRaw data quality control
üGenome assembly
üGenome component analysis
üGene function annotation
üComparative genomic analysis
For DNA extracts:
|
Sample Type |
Amount |
Concentration |
Purity |
|
DNA extracts |
＞ 3 μg |
＞ 20 ng/μl |
OD260/280= 1.6-2.5 |
For tissue samples:
| Sample type | Recommended sample treatment | Sample storage and shipment |
| Unicellular Fungus | Observe yeast under microscope and collect them in their exponential phase
Transfer the culture (containing approx. 3-4.5e9 cells) into a 1.5 or 2 ml eppendorf. (Keep on ice) Centrifuge the tube for 1 min at 14000 g to collect bacteria and remove the supernatant carefully Seal the tube and freeze the bacteria in liquid nitrogen for at least 30 min. Store the tube in -80 ℃ fridge. |
Freeze the samples in liquid nitrogen for 3-4 hours and store in liquid nitrogen or -80 degree to long-term reservation. Sample shipping with dry-ice is required. |
| Macro Fungus | Tissue in actively growing phase is recommended.
Rinse the tissue with endotoxin-free water, then 70% ethonal. Save the sample in cryo-tubes. |