Illumina pre-made libraries

Features

● Platforms: Illumina NovaSeq 6000 and NovaSeq  X Plus

● Sequencing modes: PE150 and PE250

● Quality control of libraries before sequencing

● Sequencing data QC and delivery: delivery of QC report and raw data in fastq format after demultiplexing and filtering Q30 reads

 

 

Service Advantages

Sample Platforms

Platform	Flow Cell	Sequencing mode	Unit	Estimated output
NovaSeq X	10B (8 lanes)	PE150	Single Lane Partial Lane	375Gb / Lane
	25B ( 8 lanes)	PE150	Single Lane Partial Lane	1000 Gb/Lane
NovaSeq 6000	SP Flow cell (2 lanes)	PE250	Flow Cell Single Lane Partial Lane	325-400 M reads / Lane
	S4 Flow cell (4 lanes)	PE150	Flow Cell Single Lane Partial Lane	~800 Gb / Lane

Sample Requirements

	Data Amount (X)	Concentration (qPCR/nM)	Volume
Partial Lane Sequencing	X ≤ 10 Gb	≥ 1 nM	≥ 25 μl
	10 Gb < X ≤ 50 Gb	≥ 2 nM	≥ 25 μl
	50 Gb < X ≤ 100 Gb	≥ 3 nM	≥ 25 μl
	X > 100 Gb	≥ 4 nM	≥ 25 μl
Lane Sequencing	Per Lane	≥ 1.5 nM / Library pool	≥ 25 μl / Library pool

In addition to concentration and total amount, a suitable peak pattern is also required.

Note: Lane sequencing of low diversity libraries requires PhiX spike-in to ensure robust base calling.

           We recommend submitting pre-pooled libraries as samples. If you require BMKGENE to perform library pooling, please refer to

           the library requirements for partial lane sequencing.

Library Size (Peak map)

Main peak should be within 300-450 bp.
Libraries should have a single main peak, no adapter contamination and no primer dimers.

Please reach out to us if your samples don't meet the starting material requirements.

Contact us

Service Workflow

Library quality control

Sequencing

Data quality control

Project delivery