Full-length mRNA sequencing -PacBio

Features

● cDNA synthesis from poly-A mRNA followed by library preparation

● Sequencing in CCS mode, generating HiFi reads

● Sequencing of the full-length transcripts

● The analysis does not necessitate a reference genome; however, it may be employed

● Bioinformatic analysis enables analysis of transcripts isoform lncRNA, gene fusions, poly-adenylation, and gene structure

Service Advantages

Sample Requirements and Delivery

Library	Sequencing strategy	Data recommended	Quality Control
PolyA enriched mRNA CCS library	PacBio Sequel II	20 Gb	Q30≥85%

Sample Requirements:

Nucleotides:

● Plants:

   Root, Stem or Petal: 450 mg

   Leaf or Seed: 300 mg

   Fruit: 1.2 g

● Animal:

   HEart or Intestine: 300 mg

   Viscera or Brain: 240 mg

   Muscle: 450 mg

   Bones, Hair or Skin: 1g

● Arthropods:

   Insects: 6g

   Crustacea: 300 mg

● Whole blood: 1 tube

● Cells: 10⁶ cells

 

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3.

Shipment:

1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.

2. RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

Experiment design

Sample delivery

RNA extraction

Library construction

Sequencing

Data analysis

After-sale services