- Resolution: 3.5 µM
- Spot Diameter: 2.5 µM
- Number of spots: around 4.2 Million
- 3 possible capture area formats: 6.8 mm * 6.8 mm, 11 mm * 11 mm or 15 mm * 20 mm
- H&E and fluorescent staining of sections
- Possibility to use cell segmentation technology: integration of H&E staining, fluorescent staining, and RNA sequencing to determine the limit of each cell. Processing downstream Spatial profiling analysis based on cell bin.
- Possible to achieve multilevel resolution analysis: Flexible multi-level analysis ranging from 100um to 3.5 um to resolve diverse tissue features at optimal resolution.
In the sample preparation phase, an initial bulk RNA extraction trial is performed to ensure a high-quality RNA can be obtained. In the tissue optimization stage the sections are stained and visualized and the permeabilization conditions for mRNA release from tissue are optimized. The optimized protocol is then applied during library construction, followed by sequencing and data analysis.
The complete service workflow involves real-time updates and client confirmations to maintain a responsive feedback loop, ensuring smooth project execution.