Well-designed Hi-C Sequencing - LncRNA – Biomarker

Bioinformatics

Raw data quality control Alignment with reference genome Transcript assembly Correlation analysis Known lncRNA identification Coding potential of candidate lncRNA Regulatory network: lncRNA-mRNA lncRNA expression quantification Differentially expressed lncRNA (DE-lncRNA) PCA WGCNA LncRNA target gene prediction GO and KEGG enrichment on DE-lncRNA target genes Novel lncRNA Alternative splicing prediction mRNA expression analysis Comparative analysis lncRNA vs. mRNA