Features
Two sequencing modes on PacBio sequencer: CLR and CCS.
| Mode |
Library |
Data yield |
Single-base accuracy |
Applications |
| CLR |
20Kb, 30Kb, etc. |
80 Gb to 130 Gb/cell |
Approx. 85% |
De novo, SV calling, etc. |
| CCS |
15-20 Kb |
14-40 Gb/cell (CCS) |
Approx. 99% |
De novo, Iso-Seq, SNP/Indel/SV calling |
Service Advantages
Sample Requirements
Nucleotides:
| Purity |
Appearance |
Electrophoresis Gel |
Amount |
Others |
| OD260/280=1.6-2.3;
OD260/230=1.5-2.5；
Clear peak at 260 nm |
Colourless, transparent liquid. |
Clear main band, limited degradation observed, no or limited protein, poly-saccharides or RNA contaminations |
Conc. ≥20 ng/μl; Volumn ≥15μl; Total amount depends on requirements in data yield. |
Concentration needs to be measured by Qubit and Qubit/Nanopore = 0.8-2.0 |
*NC/QC: NanoDrop concentration/Qubit concentration
Service Work Flow
Sample preparation
Library construction
Sequencing
Data analysis
Project delivery
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