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New Fashion Design for Shotgun Sequencing Cost - small RNA – Biomarker

Bioinformatics

Raw data quality control Common and unique sequence among samples Small RNA classification Alignment with reference genome (+/- strands) Genome-wide smallRNA distribution genes Known miRNA identification Small RNA annotation (miRBASE, RepBASE, Rfam, SILVA, GtRNAdb, etc.) miRNA base perference miRNA base editing miRNA family miRNA quantification Correlation analysis miRNA differential expression analysis Hierarchical clustering on DE-miRNAs miRNA target gene prediction Function annotation on DE-miRNA target genes GO and KEGG enrichment on DE-miRNA target genes

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