● rRNA depletion followed by directional mRNA library preparation.
● Sequencing on Illumina NovaSeq.
● Study the Changes of Complex Microbial Communities: This happens at the transcriptional level and explore potential new genes.
● Explaining Microbial Community Interactions with the Host or Environment.
● Comprehensive Bioinformatic Analysis: This provides insights on community taxonomic and functional compositions, as well as differential gene expression analysis.
● Extensive Gene Annotation: Using up-to-date gene function databases for informative gene expression information of microbial communities.
● Post-Sales Support: Our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.
|
Sequencing platform |
Sequencing Strategy |
Data recommended |
Data Quality Control |
|
Illumina NovaSeq |
PE150 |
12Gb |
Q30≥85% |
|
Concentration (ng/µL) |
Total amount (µg) |
Volume (µL) |
OD260/280 |
OD260/230 |
RIN |
|
≥50 |
≥1.0 |
≥20 |
1.8-2.0 |
1.0-2.5 |
≥6.5 |