● Sequencing on Illumina NovaSeq with PE150.
● Service requires tissue samples, instead of extracted nucleic acids, to cross-link with formaldehyde and conserve the DNA-protein interactions.
● The Hi-C experiment involves restriction and end repair of the sticky ends with biotin, followed by circularization of the resulting blunt ends while preserving the interactions. The DNA is then pulled down with streptavidin beads and purified for subsequent library preparation.
|
Tissue or extracted nucleic acids |
Required amount |
|
Animal Viscera |
≥ 2 g |
|
Animal Muscle |
|
|
Mammalian Blood |
≥ 2 mL |
|
Poultry/Fish Blood |
|
|
Plant- Fresh Leaf |
≥ 4 g |
|
Cultured Cells |
≥ 1x107 |