Hi-C based Chromatin Interaction

Service Features

● Sequencing on Illumina NovaSeq with PE150.

● Service requires tissue samples, instead of extracted nucleic acids, to cross-link with formaldehyde and conserve the DNA-protein interactions.

● The Hi-C experiment involves restriction and end repair of the sticky ends with biotin, followed by circularization of the resulting blunt ends while preserving the interactions. The DNA is then pulled down with streptavidin beads and purified for subsequent library preparation.

Service Advantages

● Optimal Restriction Enzyme Design: to ensure a high Hi-C efficiency on different species with up to 93% valid interaction pairs.

● Extensive Expertise and Publication Records: BMKGene has vast experience with >2000 Hi-C sequencing projects from 800 different species and various patents. Over 100 published cases with an accumulative impact factor of over 900.

● Highly-skilled Bioinformatics Team:  with in-house patents and software copyrights for Hi-C experiments and data analysis and a self-developed visualization data software.

● Post-Sales Support: Our commitment extends beyond project completion with a 3-month after-sale service period. During this time, we offer project follow-up, troubleshooting assistance, and Q&A sessions to address any queries related to the results.

● Comprehensive Annotation: we use multiple databases to functionally annotate the genes with identified variations and perform the corresponding enrichment analysis, providing insights on multiple research projects.

Service Specifications

Service Requirments