| Mode | Library | Data yield | Single-base accuracy | Applications |
| CLR | 20Kb, 30Kb, etc. | 80 Gb to 130 Gb/cell | Approx. 85% | De novo, SV calling, etc. |
| CCS | 15-20 Kb | 14-40 Gb/cell (CCS) | Approx. 99% | De novo, Iso-Seq, SNP/Indel/SV calling |
Nucleotides:
| Purity | Appearance | Electrophoresis Gel | Amount | Others |
| OD260/280=1.6-2.3； OD260/230=1.5-2.5；Clear peak at 260 nm | Colourless, transparent liquid. | Clear main band, limited degradation observed, no or limited protein, poly-saccharides or RNA contaminations | Conc. ≥20 ng/μl; Volumn ≥15μl; Total amount depends on requirements in data yield. | Concentration needs to be measured by Qubit and Qubit/Nanopore = 0.8-2.0 |