Purify high-yield, high-purity, high-quality total RNA from blood/cell/tissue.
No toxic reagents such as phenol/chloroform contained
The RNA obtained has high purity and can be directly used for chip detection, high-throughput sequencing and other experiments.
Super easy to use
No extra pipetting work.
The elution volume can be adjusted as required.
The installation and operation require little training. With pre-installed programs, unpack the cartridge, select the protocol and run your experiment. Programs are ready-to-use, and also customizable.
Preloaded reagents and matched disposable consumables.
The chemical handling is eliminated to the greatest extent to ensure the operation safety.
Agilent 2100 was used for integrity detection.
Sample source: CD-1 mice
Sample status: fresh whole blood
Sample size: 200 μl
Experiment result: the total RNA extracted from blood by TGuide S16 equipped with reagent 4993551 has good yield and high purity, and can meet the needs of downstream
high-throughput sequencing.
Sample source: CD-1 mice
Loading volume: 1 μl. The liver was diluted 5 times and loaded.
Agarose gel concentration: 1%. Electrophoresis for 20 min at 6 V/cm;
M:TIANGEN Marker III 1-4: heart 5-8:
liver 9-12: lung 13-16: kidney
The first two samples of each tissue were extracted by the spin-column based extraction kit, and the last two were extracted automatically by TGuide S16.
Experiment result: the animal tissue total RNA automatically extracted by TGuide S16 equipped with reagent 4993551 has high yield and purity, which is equivalent to the RNA extracted by the spin-column based extraction protocol. The purity is basically the same, with OD260/OD280 around 2.0~2.1 and OD260/OD230 > 2.0. In conclusion, TGuide S16 can be used to extract the total RNA from animal tissues in replacement of spin-column based extraction solution.