Eukaryotic mRNA Sequencing-NGS

Features

Advantages

Sample Requirements and Delivery

Library	Sequencing strategy	Data recommended	Quality Control
Poly A enriched	Illumina PE150 DNBSEQ-T7	6 -10 Gb	Q30≥85%

Sample Requirements:

Nucleotides:

Mimimum Sample Requirements:

Plants	Animal	Arthropods	Whole blood	Cells
Root, Stem or Petal: 450 mg Leaf or Seed: 300 mg Fruit: 1.2 g	HEart or Intestine: 300 mg Viscera or Brain: 240 mg Muscle: 450 mg Bones, Hair or Skin: 1g	Insects: 6g Crustacea: 300 mg	1 tube	106 cells

Recommended Sample Delivery

Container: 2 ml centrifuge tube (Tin foil is not recommended)

Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3... ...

Shipment:

1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.

2. RNAs table tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Sample Requirements and Delivery

Experiment design

Sample delivery

RNA extraction

Library construction

Sequencing

Data analysis

After-sale services