|
Platform |
Library Size |
Theoretical Data Yield (Per Cell) |
Single-base Accuracy |
Applications |
|
Nanopore |
8Kb, 10kb, 20kb, Ultralong, cDNA-PCR |
70-90Gb/Cell |
85-92% |
SV calling, De novo, Full-length sequencing, Iso-Seq, Gene annotation, Detection of DNA methylation |
|
Sample Type |
Amount |
Concentrtion(Qubit ®) |
Volume |
Purity |
Others |
|
Genomic DNA |
Depend on Data Requirement |
≥20ng/μl |
≥15μl |
OD260/280=1.7-2.2; OD260/230≥1.5； Clear peak at 260 nm，no contaminations |
Concentration needs to be measured by Qubit and Qubit/Nanopore ≤ 2 |
|
Total RNA |
≥1.2μg |
≥100μg/μl |
≥15μl |
OD260/280=1.7-2.5; OD260/230=0.5-2.5；no contaminations |
RIN value ≥7.5 |