● Platforms: Illumina NovaSeq 6000 and NovaSeq X Plus
● Sequencing modes: PE150 and PE250
● Quality control of libraries before sequencing
● Sequencing data QC and delivery: delivery of QC report and raw data in fastq format after demultiplexing and filtering Q30 reads
|
Platform |
Flow Cell |
Sequencing mode |
Unit |
Estimated output |
|
NovaSeq X |
10B (8 lanes) |
PE150 |
Single Lane Partial Lane |
375Gb / Lane |
|
25B ( 8 lanes) |
PE150 |
Single Lane Partial Lane |
1000 Gb/Lane |
|
|
NovaSeq 6000 |
SP Flow cell (2 lanes) |
PE250 |
Flow Cell Single Lane Partial Lane |
325-400 M reads / Lane |
|
S4 Flow cell (4 lanes) |
PE150 |
Flow Cell Single Lane Partial Lane |
~800 Gb / Lane |
|
Data Amount (X) |
Concentration (qPCR/nM) |
Volume |
|
|
Partial Lane Sequencing
|
X ≤ 10 Gb |
≥ 1 nM |
≥ 25 μl |
|
10 Gb < X ≤ 50 Gb |
≥ 2 nM |
≥ 25 μl |
|
|
50 Gb < X ≤ 100 Gb |
≥ 3 nM |
≥ 25 μl |
|
|
X > 100 Gb |
≥ 4 nM |
≥ 25 μl |
|
|
Lane Sequencing |
Per Lane |
≥ 1.5 nM / Library pool |
≥ 25 μl / Library pool |
In addition to concentration and total amount, a suitable peak pattern is also required.
Note: Lane sequencing of low diversity libraries requires PhiX spike-in to ensure robust base calling.
We recommend submitting pre-pooled libraries as samples. If you require BMKGENE to perform library pooling, please refer to
the library requirements for partial lane sequencing.
Main peak should be within 300-450 bp.
Libraries should have a single main peak, no adapter contamination and no primer dimers.