| Sequencing Mode | Library Size | Theoretical Data Yield (Per Cell) | Single-base Accuracy | Applications |
| CLR | 20Kb, 30Kb, etc. | 80 Gb to 130 Gb | Approx. 85% | De novo, SV calling, etc. |
| CCS | 15-20 Kb |
14 to 40 Gb/Cell (Sequel II) 70 to 110 Gb/Cell (Revio) Depends on the samples |
Approx. 99% | De novo, SNP/Indel/SV calling, Iso-Seq, |
|
Terms |
Sequel II system |
Revio system |
Increase |
|
Higher density |
8 million ZMWs |
25 million ZMWs |
3x |
|
Independent stages |
1 |
4 |
4x |
|
Shorter run times |
30 hours |
24 hours |
1.25x |
|
30X HiFi human genomes / year |
88 |
1,300 |
15x overall |
| Sample Type | Amount | Concentration （Qubit®） | Volume | Purity | Others |
| Genomic DNA | Depend on Data Requirement | ≥50 ng/μl | ≥15μl | OD260/280=1.7-2.2; OD260/230=1.8-2.5； Clear peak at 260 nm，no contaminations |
Concentration needs to be measured by Qubit and Qubit/Nanopore = 0.8-2.5 |
| Total RNA | ≥1.2μg | ≥120 ng/μl | ≥15μl | OD260/280=1.7-2.5; OD260/230=0.5-2.5；no contaminations |
RIN value ≥7.5 5≥28S/18S≥1 |