| Library | Sequencing strategy | Data recommended | Quality Control |
| Poly A enriched |
Illumina PE150 DNBSEQ-T7 |
6 -10 Gb |
Q30≥85% |
Nucleotides:
| Plants | Animal | Arthropods | Whole blood | Cells |
|
Root, Stem or Petal: 450 mg Leaf or Seed: 300 mg Fruit: 1.2 g |
HEart or Intestine: 300 mg Viscera or Brain: 240 mg Muscle: 450 mg Bones, Hair or Skin: 1g |
Insects: 6g Crustacea: 300 mg |
1 tube | 106 cells |
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3... ...
Shipment:
1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2. RNAs table tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
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