● With two possible options to choose from depending on the desired degree of genome completeness.
● Draft Genome Option: Short-read sequencing with Illumina NovaSeq PE150.
● Complete 0 gap genome.
● Long-read sequencing on Nanopore PromethION 48 or PacBio Revio for genome assembly.
● Short-read sequencing on Illumina NovaSeq for genome validation and error correction (Nanopore) or to generate a draft genome.
|
Concentration (ng/µL) |
Total amount (µg) |
Volume (µL) |
OD260/280 |
OD260/230 |
|
|
PacBio |
≥20 |
≥1.2 |
≥20 |
1.7-2.2 |
≥1.0 |
|
Nanopore |
≥40 |
≥2 |
≥20 |
1.7-2.2 |
1.0-3.0 |
|
Illumina |
≥1 |
≥0.06 |
≥20 |
- |
- |
· Bacteria: ≥3.5x1010 cells