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10x Genomics Visium Spatial Transcriptome

Technical Scheme

Features

● Resolution: 100 µM

● Spot Diameter: 55 µM

● Number of spots: 4992

● Capture area: 6.5 x 6.5 mm

● Each barcoded spot is loaded with primers composed of 4 sections:

    - poly(dT) tail for mRNA priming and cDNA synthesis

    - Unique Molecular Identifier (UMI) to correct amplification bias

    - Spatial barcode

    - Binding sequence of partial read 1 sequencing primer

● H&E staining of sections

Advantages

● One-stop Service:  integrates all experience and skill-based steps, including cryo-sectioning, staining, tissue optimization, spatial barcoding, library preparation, sequencing and bioinformatics .

● Highly-skilled Technical Team: with experience in over 250 tissue types and 100+ species including human, mouse, mammal, fish and plants.

● Real-time Update on the Entire Project: with full control of experimental progress.

● Comprehensive Standard Bioinformatics:  package includes 29 analyses and 100+ high-quality figures.

● Customized Data Analysis and Visualization: available for different research requests.

● Optional Joint Analysis with Single-cell mRNA Sequencing

Specifications

Service Workflow


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